Enzyme Kinetics Author : Irwin H. Offers an understanding of the behavior of enzyme systems and the diagnostic tools used to characterize them and determine kinetic mechanisms. Illustrates and explains current subjects such as cumulative, concerted and cooperative feedback inhibition and metal ion activation.
Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure.
Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level.
Enzymologists and molecular biologists will find the text a useful reference. Score: 4. Enzyme Kinetics Author : Alejandro G. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions.
It is meant to make these investigations both satisfying and effective. In distinction to other available descriptions, the descriptions in enzyme Kinetics and Mechanisms are limited to more commonly utilized and useful models and techniques. The logic relating the chemical models to the mathematical models and the logic of relating the mathematical models to data is presented in rather concise text, figures and equations.
The development of mathematical models from chemical models is done by a unique algorithm that is both simple and quick, and the same concept are utilized to develop models for the effects of a variety of reaction conditions on the initial velocity.
In addition, the various relationships of data, mathematical models and the chemical models is illustrated with examples from the scientific literature. It is primarily written for first-year research students in enzyme kinetics.
The book is composed of 10 chapters. Chapter 1 provides the basic principles of enzyme kinetics with a brief discussion of dimensional analysis. Subsequent chapters cover topics on the essential characteristics of steady-state kinetics, temperature dependence, methods for deriving steady-state rate equations, and control of enzyme activity.
Integrated rate equations, and introductions to the study of fast reactions and the statistical aspects of enzyme kinetics are provided as well. Chemists and biochemists will find the book invaluable. The content develops from monosubstrate to bisubstrate to trisubstrate reactions, concluding with nonhyperbolic rate equations and allosteric and cooperative effects.
Because it outlines the subject in such a way that it builds from less complicated to more demanding kinetic models, it can be used as a textbook for students of biochemistry and molecular biology. The author stresses the importance of graphical representation of kinetic models by frequent use of such mathematical models in the form of double-reciprocal plots. In addition, special attention is paid to isotope exchange studies, kinetic isotope effects, and the statistical evaluation of initial rate and ligand binding data.
A cursory glance at the current biochem ical literature reveals the increasing popularity of enzyme ki netics1 yet, there are very few books available to guide the enzymologist who wishes to conduct kinetic experiments.
This monograph was undertaken to provide the fledgling kineticist with an outline of contemporary initial rate enzyme kinetics. A large portion of the material contained in this book is presented in a second-year, graduate-level course in biochemistry at Iowa State University.
I have found that the presentation in this course has enabled students without a strong background in math ematics to undertake initial rate studies at the research bench. The monograph obviously is more comprehensive than any course could be, and should permit similar accomplishment.
As the title implies, the major emphasis of this monograph is on initial rate enzyme kinetics. I considered at length the advis ability of including chapters on integrated rate equations and on the theory and application of rapid reaction kinetics, such as rapid-mixing stopped-flow, and temperature-jump kinetics. These, however, are topics that would require a good deal of space to develop if they were to be helpful to the beginner. Enzyme Kinetics Author : Arthur R. Emphasis is placed on the interpretation of the kinetic behaviour of enzyme-catalyzed reactions in terms of mechanisms.
Algorithms are developed which can be implemented in computer programs for the derivation of equations. The treatment of steady-state enzyme kinetics is extended to allosteric enzymes and subunit interactions in polymeric enzymes. Other editions — View all Enzyme Kinetics: A review of significant investigations.
Theoretical aspects are presented in a way which is also comprehensible for the beginner. WileyMay 13, — Enzhme — pages. Permissions Request permission to reuse content from this site. Description Now in full color for a more intuitive learning experience, this new edition of the long-selling reference also features a number of new developments in methodology and the application of enzyme kinetics.
We use cookies to give you the best possible experience. Author information Copyright and License information Disclaimer. Principles and Methods Hans Bisswanger Limited preview — Fundamental Relationships of Enzyme Kinetics He is the author of two well-known books on enzymology that have appeared in different languages and editions. We believe that this manual has the potential to become a classic of its kind provided that the author will: The final part of the book then covers a range of widely used measurement methods and compares their performance and scope of application.
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Methods Methods for investigating multiple equilibria Electrochemical methods Calorimetry Spectroscopic methods Measurement of fast reactions show more. Measurement of enzyme activity. Moreover, the book serves in providing the reader with very useful and well-visualized data on the actual interpretation of the results that can be obtained through enzymology and the described assays; the latter is undoubtedly of high importance, as firm interpretation of any performed enzymatic assay is equally important to its methodological accuracy and vice versa.
Please review our privacy policy. An extensive methodological part provides the expert with valuable support in planning bisswxnger performing laboratory experiments. Starting with a description of ligand binding equilibria, the experienced author goes on to discuss simple and complex enzyme reactions in kinetic terms. Advances in enzyme technology: Special cases such as membrane-bound and immobilized enzymes are considered, as is the influence of external conditions, such as temperature and pH value.
This is a user-friendly and comprehensive treatise on enzyme kinetics — indispensable for biochemists, biologists, medical scientists, and chemists working with enzymes, from advanced students to experts in academia and industry. Adv Biochem Eng Biotechnol.
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